Methylmalonyl Coenzyme A mutase Mouse Monoclonal Antibody

SKU: orb654292

Description

Anti-Methylmalonyl Coenzyme A mutase Antibody (monoclonal, 2D6). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Research Area

Signal Transduction

Images & Validation

• Tested Applications: IHC, WB
• Dilution Range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2b
• Clone No.: 2D6
• Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human MUT, different from the related mouse sequence by one amino acid.
• Target: Methylmalonyl-CoA mutase, mitochondrial
• Molecular Weight: 83 kDa
• Purification: Immunogen affinity purified.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Concentration: 500 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

MCM; Methylmalonyl CoA isomerase; MUT

IHC analysis of Methylmalonyl Coenzyme A using anti-Methylmalonyl Coenzyme A antibody. Methylmalonyl Coenzyme A was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml mouse anti-Methylmalonyl Coenzyme A Antibody overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

IHC analysis of Methylmalonyl Coenzyme A using anti-Methylmalonyl Coenzyme A antibody. Methylmalonyl Coenzyme A was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml mouse anti-Methylmalonyl Coenzyme A Antibody overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

IHC analysis of Methylmalonyl Coenzyme A using anti-Methylmalonyl Coenzyme A antibody. Methylmalonyl Coenzyme A was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml mouse anti-Methylmalonyl Coenzyme A Antibody overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

IHC analysis of Methylmalonyl Coenzyme A using anti-Methylmalonyl Coenzyme A antibody. Methylmalonyl Coenzyme A was detected in paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml mouse anti-Methylmalonyl Coenzyme A Antibody overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Western blot analysis of Methylmalonyl Coenzyme A using anti-Methylmalonyl Coenzyme A antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates; Lane 2: human K562 whole cell lysates; Lane 3: human HEK293 whole cell lysates; Lane 4: human PC-3 whole cell lysates; Lane 5: human Caco-2 whole cell lysates; Lane 6: human Raji whole cell lysates; Lane 7: rat PC-12 whole cell lysates; Lane 8: mouse NIH/3T3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Methylmalonyl Coenzyme A antigen affinity purified monoclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Methylmalonyl Coenzyme A at approximately 83 KD. The expected band size for Methylmalonyl Coenzyme A is at 83 KD.

Quick Database Links

Gene Symbol

Methylmalonyl-CoA mutase, mitochondrial

UniProt

P22033