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Anti-Lysozyme LYZ Rabbit Monoclonal Antibody
Catalog Number: orb547865
| Catalog Number | orb547865 |
|---|---|
| Category | Antibodies |
| Description | Anti-Lysozyme LYZ Rabbit Monoclonal Antibody. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat. |
| Species/Host | Rabbit |
| Clonality | Monoclonal |
| Clone Number | EBO-12 |
| Tested applications | ICC, IF, IHC, IP, WB |
| Reactivity | Human, Mouse, Rat |
| Isotype | Rabbit IgG |
| Immunogen | A synthesized peptide derived from human Lysozyme |
| Antibody Type | Primary Antibody |
| Concentration | Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure. |
| Form/Appearance | Liquid |
| Conjugation | Unconjugated |
| MW | 15 kDa |
| UniProt ID | P61626 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | Lysozyme C; 3.2.1.17; 1, 4-beta-N-acetylmuramidase Read more... |
| Note | For research use only |
| Application notes | WB 1:5000-1:10000IHC 1:50-1:200ICC/IF 1:50-1:200IP 1:20. Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL |
| Expiration Date | 12 months from date of receipt. |
Immunohistochemical analysis of paraffin-embedded mouse kidney, using Lysozyme Antibody.
Western blot analysis of Lysozyme using anti-Lysozyme antibody (orb547865). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: human U937 whole cell lysates, Lane 3: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Lysozyme antigen affinity purified monoclonal antibody (Catalog # orb547865) at 1:5000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # orb90502) with Tanon 5200 system. A specific band was detected for Lysozyme at approximately 15 kDa. The expected band size for Lysozyme is at 17 kDa.
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