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Item 1 of 4
Item 1 of 4
Anti-LAT Purified
Catalog Number: orb43896
| Catalog Number | orb43896 |
|---|---|
| Category | Antibodies |
| Description | Mouse Monoclonal to LAT. |
| Target | LAT |
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Concentration | 1 mg/ml |
| Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Purity | Purified by protein-A affinity chromatography. |
| Purification | Purified by protein-A affinity chromatography. |
| Immunogen | Bacterially produced recombinant polypeptide corresponding to the entire cytoplasmic domain of human LAT. |
| UniProt ID | O43561 |
| RRID | AB_10996537 |
| Tested applications | FC, IP, WB |
| Application notes | Immunoprecipitation: Tyrosine-phosphorylated form of LAT is not optimally recognized. Flow cytometry: Intracellular staining. Tyrosine-phosphorylated form of LAT is not optimally recognized.Western blotting: Recommended dilution: 1-2 μg/ml. |
| Antibody Type | Primary Antibody |
| Clone Number | LAT-01 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | Anti-LAT antibody |
| Note | For research use only |
| Entrez | 27040 |
Flow cytometry multicolor intracellular staining of human peripheral whole blood stained using anti-LAT (LAT-01) purified antibody (concentration in sample 1 µg/ml, GAM APC) and anti-human CD3 (UCHT1) Pacific Blue™ antibody (20 µl reagent / 100 µl of peripheral whole blood).
Separation of human CD3 positive LAT positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (intracellular staining) of peripheral whole blood stained using anti-LAT (LAT-01) purified antibody (concentration in sample 1 µg/ml, GAM APC).
Flow cytometry intracellular staining pattern of human peripheral whole blood using anti-LAT (LAT-01) purified antibody (concentration in sample 1 µg/ml, GAM APC).
Western blotting analysis of human LAT using mouse monoclonal antibody LAT-01 in lysates of Jurkat cells (positive) and Raji cells (negative control) under non-reducing and reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-LAT monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. A specific band was detected for LAT at approximately 38 kDa.
