KSR2 Antibody

SKU: orb2650101

Description

Rabbit polyclonal antibody to KSR2

Images & Validation

• Tested Applications: IF, IHC, IP, WB
• Dilution Range: WB: WB (1/500 - 1/1000), IH (1/50 - 1/100), IF/IC (1/100 - 1/500), IP (1/10 - 1/100), IF: WB (1/500 - 1/1000), IH (1/50 - 1/100), IF/IC (1/100 - 1/500), IP (1/10 - 1/100)
• Reactivity: Human, Mouse

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Clone No.: KSR2
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Kinase suppressor of Ras 2; hKSR2

Western blot analysis of KSR2 expression in Hela (A), HEK293T (B), A549 (C), mouse brain (D) whole cell lysates.

Immunohistochemical analysis of KSR2 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of KSR2 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4°C in a hidified chamber. Cells were washed with PBST and incubated with Alexa Fluor 647-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Quick Database Links

UniProt

Q6VAB6