CNKSR2/KSR2 Rabbit Polyclonal Antibody

SKU: orb2283

Description

CNKSR2/KSR2 Rabbit Polyclonal Antibody

Research Area

Cell Biology, Cytoplasmic, Ras Family, Signal Transduction

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Gallus, Porcine, Rabbit

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human CNKSR2 (481-580/1034aa)
• Target: CNKSR2
• Molecular Weight: 114 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CNK2; KSR2; MAGUIN; MRXSHG; CNKR2_HUMAN; CNKSR2; Connector enhancer of KSR 2; CNK homolog protein 2 (CNK2); KIAA0902; CNKR2_MOUSE; CNKR2_RAT; Membrane-associated guanylate kinase-interacting protein (Maguin);

Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CNKSR2/KSR2) Polyclonal Antibody, Unconjugated (orb2283) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CNKSR2/KSR2) Polyclonal Antibody, Unconjugated (orb2283) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: U251 (Human) Cell Lysate at 30 ug, Primary: Anti-CNKSR2/KSR2 (orb2283) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 114 kD, Observed band size: 114 kD.

Quick Database Links

Gene Symbol

CNKSR2

UniProt

Q8WXI2