Anti-Interleukin-10 IL10 Antibody

SKU: orb2580357

Description

Anti-Interleukin-10 IL10 Antibody. Tested in ELISA, WB applications. This antibody reacts with Mouse.

Images & Validation

• Tested Applications: FC
• Reactivity: Mouse
• Application Notes:
  Flow Cytometry, 1-3μg/1x106 cells. Add 0.2ml of distilled water will yield a concentration of 500ug/ml

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: E. coli-derived mouse IL-10 recombinant protein (Position: S19-S178).
• Target: Interleukin-10(IL-10)
• Molecular Weight: IL10 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IL10 Antibody (RP1014) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
• Purification: Immunogen affinity purified.
• Conjugation: FITC

Storage & Handling

• Storage: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
• Form/Appearance: Liquid
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Disclaimer: For research use only

Alternative Names

Interleukin-10; IL-10; Cytokine synthesis inhibitory factor; CSIF; Il10; Il-10

Quick Database Links

Gene Symbol

Interleukin-10(IL-10)

UniProt

P18893