Anti-IL-5 Reference Antibody (mepolizumab)

• Catalog Number: orb1818437
• Category: Antibodies
• Description: Recombinant Antibody
• Clonality: Monoclonal
• Tested applications: ELISA, FC
• Reactivity: Human
• Isotype: IgG1
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Form/Appearance: Purified monoclonal antibody supplied in PBS, pH 6.0, without preservative.This antibody is purified through a protein A column.
• Conjugation: Unconjugated
• MW: 146.24
• UniProt ID: P05113
• Protein Sequence: IL-5
• Expression System: CHO Cell
• Endotoxins: < 0.001EU/μg,determined by LAL method.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Interleukin-5, IL-5, B-cell differentiation factor
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

The purity of Anti-IL-5 Reference Antibody (mepolizumab) is more than 98.47%, determined by SEC-HPLC.

Immobilized human IL 5 His at 2 µg/mL can bind Anti-IL-5 Reference Antibody (mepolizumab), EC50=0.1188 µg/mL

Mepolizumab induced proliferation inhibition activity was evaluated using Human IL-5Rα TF-1.The EC50 was approximately 0.3282 ug/mL.

Anti-IL-5 Reference Antibody (mepolizumab) FACS Blocking was evaluated using Human IL-5Rβ-FL/HIL-5Rα CHOS. The IC50 was approximately 2.844 ug/mL.

Anti-IL-5 Reference Antibody (mepolizumab) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%