Anti-IL-5 Reference Antibody (mepolizumab)

• Catalog Number: orb1806163
• Category: Antibodies
• Description: Anti-IL-5 Reference Antibody is expressed from CHO. The heavy chain type is huIgG1, and the light chain type is hukappa. It has a predicted MW of 145.5 kDa.
• Clonality: Monoclonal
• Tested applications: ELISA, FA, FACS, Kinetics
• Reactivity: Human
• Isotype: IgG
• Purity: > 95%
• Conjugation: Unconjugated
• MW: 145.5 kDa
• Target: IL-5
• Endotoxins: < 1 EU/mg
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 100 mM Pro-Ac 20 mM Arg pH 5.0
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Anti-IL-5 Reference Antibody (mepolizumab) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

The purity of Anti-IL-5 Reference Antibody (mepolizumab)is more than 98.47% ;determined by SEC-HPLC.

Immobilized human IL 5 His at 2 μg/mL can bind Anti-IL-5 Reference Antibody (mepolizumab);EC50=0.1188μg/mL.

Anti-IL-5 Reference Antibody (mepolizumab) FACS Blocking was evaluated using Human IL-5Rβ-FL/HIL-5Rα CHOS. The IC50 was approximately 2.844 ug/mL.

Mepolizumab induced proliferation inhibition activity was evaluated using Human IL-5Rα TF-1 . The EC50 was approximately 0.3282 ug/mL.