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Item 1 of 3
Anti-Hu CD206 Purified Low Endotoxin
Catalog Number: orb308746
| Catalog Number | orb308746 |
|---|---|
| Category | Antibodies |
| Description | Mouse monoclonal antibody to CD206 |
| Clonality | Monoclonal |
| Clone Number | 15-2 |
| Tested applications | FA, FC, ICC, IHC-Fr, IP, WB |
| Reactivity | Human |
| Isotype | Mouse IgG1 kappa |
| Immunogen | Purified human mannose receptor |
| Antibody Type | Primary Antibody |
| Concentration | 1 mg/ml |
| Dilution range | Functional application: Blocking. Flow cytometry: Recommended dilution: 1-4 µg/ml. Western blotting: Recommended dilution: 1-2 µg/ml. |
| Purity | Purified by protein-A affinity chromatography. |
| Conjugation | Unconjugated |
| Target | CD206 |
| Entrez | 4360 |
| UniProt ID | P22897 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4 |
| Alternative names | Anti-MMR antibody, anti-MRC1 antibody, anti-CLEC13 Read more... |
| Note | For research use only |
| Application notes | Low Endotoxin, Azide free format. Product does not contain preservatives and is intended primarily for functional applications. Preservative containing buffers are available for IHC applications |
| Expiration Date | 12 months from date of receipt. |
Separation of human CD206 positive dendritic cells differentiated upon monocyte stimulation (GM-CSF + IL-4) (red-filled) from non-stimulated lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human stimulated (GM-CSF + IL-4) peripheral blood mononuclear cells stained using anti-human CD206 (15-2) purified antibody (low endotoxin, concentration in sample 9 μg/ml), GAM PE.
Flow cytometry surface staining pattern of human stimulated (GM-CSF + IL-4) peripheral blood mononuclear cells stained using anti-human CD206 (15-2) purified antibody (low endotoxin, concentration in sample 9 μg/ml), GAM PE.
Western blotting analysis of human CD206 using mouse monoclonal antibody 15-2 on lysates of differentiated monocytes (two batches), and on THP-1 and Raji cells (negative controls) under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. CD206 was detected around 170 kDa.
