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Item 1 of 2
Anti-HMG-20A Antibody
Catalog Number: orb341265
| Catalog Number | orb341265 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody to HMG20A |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | IF, WB |
| Reactivity | Human, Mouse |
| Immunogen | Recombinant full length protein of human HMG-20A |
| Antibody Type | Primary Antibody |
| Dilution range | WB: 1:500-1:2000, IHC-P: 1:50-1:200, IF/ICC: 1:50-1:100 |
| Form/Appearance | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Conjugation | Unconjugated |
| Target | HMG20A |
| Entrez | 10363, 66867 |
| UniProt ID | Q9DC33, Q9NP66 |
| Source | Rabbit |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Alternative names | HMGX1; HMGXB1; High mobility group protein 20A; HM Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Western blot analysis of HMG-20A expression in HEK293T (A), Hela (B) whole cell lysates. (Predicted band size: 8; 40 kD; Observed band size: 41 kD)
Immunofluorescent analysis of HMG-20A staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
