HLA-C Rabbit Polyclonal Antibody

SKU: orb411674

Description

Rabbit polyclonal antibody to HLA-C

Images & Validation

• Tested Applications: IF, WB
• Dilution Range: WB: 1:500-2000
• Reactivity: Human, Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein of human HLA-C
• Target: HLA-C
• Purification: The antibody was purified by immunogen affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti-HLAC antibody, anti-HLA class I histocompatibility antigen, Cw-1 alpha chain antibody, anti-MHC class I antigen Cw*1 antibody

Western blot analysis of HLA-C expression in HL60 (A), Jurkat (B), mouse liver (C) whole cell lysates. (Predicted band size: 41 kD; Observed band size: 41 kD)

Immunofluorescent analysis of HLA-C staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Quick Database Links

Gene Symbol

HLA-C

UniProt

P30499

NCBI Gene ID

3107