Anti-Haemoglobin [2A1]

SKU: orb1089969

Description

Rabbit monoclonal antibody to Haemoglobin

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: ELISA, WB
• Reactivity: Mouse

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG
• Clone No.: 2A1
• Immunogen: Murine hybridomas were generated using spleen cells from aging (8–10 month old) NZB/W F1 animals. Hybridomas secreting antibodies reactive to murine hemoglobin were subcloned by limiting dilution and antibody isotypes determined using commercial reagents. Eight-week-old NZB/W F1 were immunized subcutaneously with 50 mg mHb antigen emulsified in Complete Fruend’s Adjuvant.
• Target: Haemoglobin
• Purity: Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Hemoglobin; Hb

Immunofluorescence staining of fixed mouse splenocytes with anti-Haemoglobin antibody 2A1. Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes on Shi-fix™ coverslips, permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of 2A1 (orb1089969) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb1089969, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256449) followed by staining with Alexa Fluor® 488 secondary antibody.

Quick Database Links

Gene Symbol

Haemoglobin

UniProt

P01942