Anti-GPR54/KISS1R Antibody

• Catalog Number: orb2599870
• Category: Antibodies
• Description: Anti-GPR54/KISS1R Antibody. Tested in ELISA, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: PE
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Lyophilized
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Buffer/Preservatives: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4
• Immunogen: E.coli-derived human GPR54/KISS1R recombinant protein (Position: N60-A373).
• UniProt ID: Q969F8
• MW: Observed: 80 kDa
• Tested applications: ELISA, ICC, IF, IHC, WB
• Dilution range: Western blot, 0.25-0.5 μg/ml, Immunohistochemistry(Paraffin-embedded Section), 1-2 μg/ml, Immunocytochemistry/Immunofluorescence, 5 μg/ml, ELISA, 0.1-0.5 μg/ml
• Application notes: WB: human MCF-7 whole cell, human Hela whole cell, human HepG2 whole cell, rat kindey tissue, mouse pancreas tissue, mouse kindey tissue. IHC: human gall bladder adenosquamous carcinoma tissue, human hyroid papillary carcinoma tissue, human liver cancer tissue, mouse colon tissue, mouse brain tissue, rat brain tissue. ICC/IF: CACO-2 cell
• Antibody Type: Primary Antibody
• Storage: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
• Alternative names: KiSS-1 receptor; KiSS-1R; G-protein coupled recept
• Background: The KiSS1-derived peptide receptor (also known as
• Note: For research use only

Western blot analysis of GPR54/KISS1R using antiGPR54/KISS1R antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: rat kindey tissue lysates, Lane 5: mouse pancreas tissue lysates, Lane 6: mouse kindey tissue lysates.

IHC analysis of GPR54/KISS1R using anti-GPR54/KISS1R antibody. GPR54/KISS1R was detected in a paraffin-embedded section of human gall bladder adenosquamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GPR54/KISS1R Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen

IHC analysis of GPR54/KISS1R using anti-GPR54/KISS1R antibody. GPR54/KISS1R was detected in a paraffin-embedded section of human hyroid papillary carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GPR54/KISS1R Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen

IHC analysis of GPR54/KISS1R using anti-GPR54/KISS1R antibody. GPR54/KISS1R was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GPR54/KISS1R Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen

IHC analysis of GPR54/KISS1R using anti-GPR54/KISS1R antibody. GPR54/KISS1R was detected in a paraffin-embedded section of mouse colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GPR54/KISS1R Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen

IHC analysis of GPR54/KISS1R using anti-GPR54/KISS1R antibody. GPR54/KISS1R was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GPR54/KISS1R Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen

IHC analysis of GPR54/KISS1R using anti-GPR54/KISS1R antibody. GPR54/KISS1R was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GPR54/KISS1R Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen

IF analysis of GPR54/KISS1R using anti-GPR54/KISS1R antibody. GPR54/KISS1R was detected in an immunocytochemical section of CACO-2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-GPR54/KISS1R Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used