Anti-GFP Tag (Goat Polyclonal)

SKU: orb1140400

Description

Goat polyclonal antibody to GFP Tag

Images & Validation

• Tested Applications: ELISA, ICC, IF, IHC, IP, WB
• Dilution Range: ELISA 1:40,000; WESTERN BLOT 1:400 - 1:2,000; IMMUNOHISTOCHEMISTRY 1:200 - 1:1,000; IF MICROSCOPY 1:1,000; IP 1:200
• Reactivity: Other
• Application Notes:
  ELISA 1:40,000; WESTERN BLOT 1:400 - 1:2,000; IMMUNOHISTOCHEMISTRY 1:200 - 1:1,000; IF MICROSCOPY 1:1,000; IP 1:200

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: The anti-GFP immunogen is a Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and purified and partially purified Green Fluorescent Protein (Aequorea victoria). No reaction was observed against Human, Mouse or Rat serum proteins.
• Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and purified and partially purified Green Fluorescent Protein (Aequorea victoria). No reaction was observed against Human, Mouse or Rat serum proteins.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS, pH 7.4 with 0.05% sodium azide.
• Concentration: 100ug/100ul
• Disclaimer: For research use only