Anti-Fibronectin [BC-1]

• Catalog Number: orb2645787
• Category: Antibodies
• Description: Anti-Fibronectin [BC-1]
• Target: Fibronectin
• Clonality: Monoclonal
• Species/Host: Human
• Isotype: IgG1
• Conjugation: Unconjugated
• Reactivity: Human
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Immunogen: The original mouse antibody was generated by immunizing mice with fibronectin derived from a culture medium of SV40-transformed Wi-38 embryonic human lung cells and defined Wi-38Val3. The humanized version was generated later by grafting CDRs of original mouse antibody onto human framework regions.
• UniProt ID: P02751
• Tested applications: IHC, RIA, WB
• Clone Number: BC-1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: FN; EDB(+) FN; ED-B domain of fibronectin; Extra d
• Note: For research use only

Immunofluorescence staining of HepG2 cells with anti-Fibronectin antibody BC-1. Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells stained with the chimeric rabbit IgG version of BC-1 (orb2645786) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, orb2645786, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.