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Anti-FDCSP Antibody
Catalog Number: orb18784
| Catalog Number | orb18784 |
|---|---|
| Category | Antibodies |
| Description | Anti-FDCSP Antibody |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Isotype | Rabbit IgG |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Form/Appearance | Lyophilized |
| Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human FDCSP. |
| UniProt ID | Q8NFU4 |
| MW | 28 kDa |
| Tested applications | IF, IHC |
| Application notes | Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human Immunofluorescence, 5 μg/ml, Human. Add 0.2ml of distilled water will yield a concentration of 500ug/ml |
| Antibody Type | Primary Antibody |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | Follicular dendritic cell secreted peptide; FDC se Read more... |
| Note | For research use only |
IF analysis of FDCSP using anti-FDCSP antibody. FDCSP was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 µg/mL rabbit anti-FDCSP Antibody overnight at 4°C. FITC Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
IHC analysis of FDCSP using anti-FDCSP antibody. FDCSP was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml rabbit anti-FDCSP Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
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