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Anti-EIF3A Antibody

Catalog Number: orb378044

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb378044
CategoryAntibodies
DescriptionRabbit polyclonal antibody to EIF3A.
TargetEIF3A
ClonalityPolyclonal
Species/HostRabbit
ConjugationUnconjugated
ReactivityHuman, Mouse
Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ImmunogenRecombinant full length protein of human EIF3A
UniProt IDQ14152, P23116
Tested applicationsIF, WB
Dilution rangeWB: 1:500-1000, IHC-P: 1:50-200
Antibody TypePrimary Antibody
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesEIF3S10; KIAA0139; Eukaryotic translation initiati
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NoteFor research use only
Entrez13669, 8661
Anti-EIF3A Antibody

Western blot analysis of EIF3A expression in HepG2 (A), Jurkat (B), mouse heart (C), mouse ovary (D) whole cell lysates. (Predicted band size: 162; 166 kD; Observed band size: 180 kD)

Anti-EIF3A Antibody

Immunofluorescent analysis of EIF3A staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

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    IP,  WB

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    IP,  WB

    Human, Mouse

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