E74 like factor 1/ELF1 Rabbit Polyclonal Antibody

SKU: orb402284

Description

Anti-E74 like factor 1/ELF1 Antibody. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Research Area

Epigenetics & Chromatin

Images & Validation

• Tested Applications: WB
• Dilution Range: Western blot, 0.1-0.5μg/ml
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human E74 like factor 1, which shares 81.6% amino acid (aa) sequence identity with both mouse and rat E74 like factor 1.
• Target: ETS-related transcription factor Elf-1
• Molecular Weight: 75 kDa
• Purification: Immunogen affinity purified.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

E74 like factor 1; ELF1

Western blot analysis of E74 like factor 1 using anti-E74 like factor 1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human COLO-320 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human PANC-1 whole cell lysates, Lane 5: Human A431 whole cell lysates, Lane 6: human Jurkat whole cell lysates, Lane 7: human 293T whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-E74 like factor 1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for E74 like factor 1 at approximately 75KD. The expected band size for E74 like factor 1 is at 67KD.

Quick Database Links

Gene Symbol

ETS-related transcription factor Elf-1

UniProt

P32519