Anti-CHAT Antibody

• Catalog Number: orb1474634
• Category: Antibodies
• Description: Rabbit polyclonal antibody to CHAT
• Target: CHAT
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: Recombinant fusion protein of human CHAT. The exact sequence is proprietary.
• UniProt ID: P32738, Q03059, P28329
• Tested applications: IF, WB
• Dilution range: WB (1/500 - 1/1000), IF/IC (1/50 - 1/100)
• Antibody Type: Primary Antibody
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Choline O-acetyltransferase; CHOACTase; ChAT; Chol
• Note: For research use only
• Entrez: 1103

Western blot analysis of CHAT expression in HepG2 (A), HeLa (B), C6 (C) whole cell lysates. (Predicted band size: 70 kD; Observed band size: 70 kD)

Immunofluorescent analysis of Integrin alpha 7 staining in rat brain. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).