Anti-CD48 [OX-78]

SKU: orb2645690

Description

Anti-CD48 [OX-78]

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: Blocking, FC, IP
• Reactivity: Mouse

Key Properties

• Host: Rat
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone No.: OX-78
• Immunogen: OX-78 was prepared by immunising rats against mouse CD48.
• Target: CD48
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

BCM1; BCM1 surface antigen; BLAST1; BLAST-1; HM48-1; MRC OX-45 surface antigen; MRC OX45 surface antigen; OX45 surface antigen; OX-45 surface antigen; SLAM family member 2; sgp60; sgp-60; OX78; MRC OX-78; MRC OX78

Immunofluorescence staining of fixed mouse thymocytes with anti-CD48 antibody OX-78. Immunofluorescence analysis of paraformaldehyde fixed mouse (Mus musculus) thymocytes on Shi-fix™ coverslips, permeabilized with 0.15% Triton stained with the chimeric rabbit version of OX-78 (orb2645691) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 µg/ml), showing membrane staining in a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb2645691, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor® 488 secondary antibody.

Western Blot using anti-CD48 antibody OX-78 Mouse spleen (A), thymus (B) and lymph node (C) tissue lysate samples (35 µg protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of OX-78 (orb2645691) at 0.0001 µg/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected running size for CD48 is ~37-56kDa and it is heavily glycosylated, as well as lipidated (UniProt ID: P18181). orb2645691 successfully detected CD48 in mouse spleen, thymus and lymph node tissue lysates.

Quick Database Links

Gene Symbol

CD48

UniProt

P18181