Anti-CD40 [A54 scFv]

SKU: orb613917

Description

Rabbit monoclonal antibody to CD40

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: Blocking, ELISA
• Reactivity: Human

Key Properties

• Host: Human
• Clonality: Monoclonal
• Isotype: Human IgG
• Clone No.: A54 scFv
• Immunogen: A54 human anti-CD40 scFv was selected from the n-CoDeR phage display library after 4 rounds of selections using biotinylated CD40-Fcγ fusion protein as bait and subsequent capture of bound phages on streptavidin-caoted magnetic beads.
• Target: CD40
• Purity: Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

TNFRSF5; A54; Tumor necrosis factor receptor superfamily member 5; B cell surface antigen CD40; B-cell cell surface antigen CD40; B-cell surface antigen CD40; B cell surface antigen CD40; antigen CD40; CD40 antigen; Bp50; Bp-50; CD40L receptor CDw40

Immunofluorescence staining of fixed Daudi cells with anti-CD40 antibody A54 scFv. Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix™ coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of A54 scFv (orb613917) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb613917, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor® 488 secondary antibody.

Quick Database Links

Gene Symbol

CD40

UniProt

P25942