Anti-CD169 [SER-4 (recombinant version)]

SKU: orb2645759

Description

Anti-CD169 [SER-4 (recombinant version)]

Research Area

Cell Biology, Immunology & Inflammation

Images & Validation

• Tested Applications: IHC
• Reactivity: Mouse

Key Properties

• Antibody Type: Recombinant Antibody
• Host: Rat
• Clonality: Monoclonal
• Isotype: IgG
• Clone No.: SER-4 (recombinant version)
• Immunogen: Spleen cells from AO rats immunised with thioglycollate-elicted murine peritoneal macrophages (TPM) that had been cutured in mouse serum to induce sheep erythrocyte receptor (SER).
• Target: CD169
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Sialoadhesin; Sheep erythrocyte receptor; SER; Sialic acid-binding Ig-like lectin 1; Siglec-1

Flow cytometry using the Anti-CD169 antibody SER-4 (recombinant version). Paraformaldehyde fixed RAW264.7 cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG version of SER-4 (recombinant version) (orb2645759, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of RAW264.7 cells with anti-CD169 SER-4 (recombinant version). Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells stained with the chimeric rabbit IgG version of SER-4 (recombinant version) (orb2645759) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb2645759, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.

Quick Database Links

Gene Symbol

CD169

UniProt

Q62230