CD146/MCAM Mouse Monoclonal Antibody

SKU: orb654279

Description

The CD146/MCAM Mouse Monoclonal Antibody is an unconjugated antibody raised in mouse. It is immunogen affinity purified and suitable for use in FC, IHC, IHC-Fr, and WB applications. This antibody targets the Cell surface glycoprotein MUC18 in Human samples.

Research Area

Cell Biology, Immunology & Inflammation, Signal Transduction

Images & Validation

• Tested Applications: FC, IHC, IHC-Fr, WB
• Dilution Range: Western blot, 0.1-0.5μg/ml, Human Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human Flow Cytometry (Fixed), 1-3μg/1x10^6 cells, Human
• Reactivity: Human
• Application Notes:
  For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2a
• Clone No.: B6E34
• Immunogen: E.coli-derived human CD146 recombinant protein (Position: H59-A401). Human CD146 shares 73% amino acid (aa) sequence identity with both mouse and rat CD146.
• Target: Cell surface glycoprotein MUC18
• Molecular Weight: 120 kDa
• Purification: Immunogen affinity purified.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Concentration: 500 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CD146; MCAM; MUC18

Quick Database Links

Gene Symbol

Cell surface glycoprotein MUC18

UniProt

P43121