CATSPER1 Antibody

• Catalog Number: orb234792
• Category: Antibodies
• Description: The CATSPER1 Antibody is suitable for IF, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CATSPER1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Monkey, Mouse, Rat
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CATSPER1. The exact sequence is proprietary.
• UniProt ID: Q8NEC5, Q91ZR5
• Tested applications: IF, WB
• Dilution range: WB: 1:500-1000
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Cation channel sperm-associated protein 1; CatSper
• Research Area: Developmental Biology
• Note: For research use only
• Entrez: 117144, 225865

References

Jing Gu 1, Ning Zhang 1, Xiao Jiang 1, Lei Zhu 1, Yixia Lou 1, Shengqi Sun 1, Li Yin 1 2, Jinyi Liu 1 The Olfactory Receptor Olfr25 Mediates Sperm Dysfunction Induced by Low-Dose Bisphenol A through the CatSper-Ca2+ Signaling Pathway Toxics, 12, 442 (2024)

PubMed: 38922122

Applications

WB

Reactivity

Mouse

Western blot analysis of CATSPER1 expression in Hela (A), DLD (B), A375 (C), mouse testis (D), rat testis (E) whole cell lysates. (Predicted band size: 90 kD; Observed band size: 110 kD)

Immunofluorescent analysis of CATSPER1 staining in SGC7901 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).