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Catalog Number | orb234792 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to CATSPER1 |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, WB |
Reactivity | Human, Mouse, Primate, Rat |
Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CATSPER1. The exact sequence is proprietary. |
Antibody Type | Primary Antibody |
Dilution range | WB: 1:500-1000 |
Form/Appearance | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Conjugation | Unconjugated |
Target | CATSPER1 |
Entrez | 225865, 117144 |
UniProt ID | Q91ZR5, Q8NEC5 |
Source | Rabbit |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative names | anti Cation channel sperm-associated protein 1 ant Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
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Jing Gu 1, Ning Zhang 1, Xiao Jiang 1, Lei Zhu 1, Yixia Lou 1, Shengqi Sun 1, Li Yin 1 2, Jinyi Liu 1 The Olfactory Receptor Olfr25 Mediates Sperm Dysfunction Induced by Low-Dose Bisphenol A through the CatSper-Ca2+ Signaling Pathway Toxics, 12, 442 (2024)
Applications
Reactivity
Western blot analysis of CATSPER1 expression in Hela (A), DLD (B), A375 (C), mouse testis (D), rat testis (E) whole cell lysates. (Predicted band size: 90 kD; Observed band size: 110 kD)
Immunofluorescent analysis of CATSPER1 staining in SGC7901 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).