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Anti-CATSPER1 Antibody

Catalog Number: orb234792

DispatchUsually dispatched within 5-10 working days
$ 140.00
Catalog Numberorb234792
CategoryAntibodies
DescriptionRabbit polyclonal antibody to CATSPER1
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, WB
ReactivityHuman, Mouse, Primate, Rat
ImmunogenKLH-conjugated synthetic peptide encompassing a sequence within the center region of human CATSPER1. The exact sequence is proprietary.
Antibody TypePrimary Antibody
Dilution rangeWB: 1:500-1000
Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ConjugationUnconjugated
TargetCATSPER1
Entrez225865, 117144
UniProt IDQ91ZR5, Q8NEC5
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative namesanti Cation channel sperm-associated protein 1 ant
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NoteFor research use only
Expiration Date12 months from date of receipt.

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Jing Gu 1, Ning Zhang 1, Xiao Jiang 1, Lei Zhu 1, Yixia Lou 1, Shengqi Sun 1, Li Yin 1 2, Jinyi Liu 1 The Olfactory Receptor Olfr25 Mediates Sperm Dysfunction Induced by Low-Dose Bisphenol A through the CatSper-Ca2+ Signaling Pathway Toxics, 12, 442 (2024)

Applications

WB

Reactivity

Mouse
Anti-CATSPER1 Antibody

Western blot analysis of CATSPER1 expression in Hela (A), DLD (B), A375 (C), mouse testis (D), rat testis (E) whole cell lysates. (Predicted band size: 90 kD; Observed band size: 110 kD)

Anti-CATSPER1 Antibody

Immunofluorescent analysis of CATSPER1 staining in SGC7901 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

  • CATSPER1 peptide [orb234423]

    Blocking

    > 85%

    Synthetic

    5 mg, 1 mg
  • Anti-CATSPER1 Antibody [orb1422941]

    WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    100 μl, 50 μl