CATSPER1 Antibody

SKU: orb234792

Description

The CATSPER1 Antibody is suitable for IF, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CATSPER1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.

Research Area

Developmental Biology

Images & Validation

• Tested Applications: IF, WB
• Dilution range: WB: 1:500-1000
• Reactivity: Human, Monkey, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CATSPER1. The exact sequence is proprietary.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Disclaimer: For research use only

Alternative Names

Cation channel sperm-associated protein 1; CatSper1; hCatSper

Western blot analysis of CATSPER1 expression in Hela (A), DLD (B), A375 (C), mouse testis (D), rat testis (E) whole cell lysates. (Predicted band size: 90 kD; Observed band size: 110 kD)

Immunofluorescent analysis of CATSPER1 staining in SGC7901 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Quick Database Links

UniProt

Q8NEC5, Q91ZR5

NCBI Gene ID

225865, 117144

References

Jing Gu 1, Ning Zhang 1, Xiao Jiang 1, Lei Zhu 1, Yixia Lou 1, Shengqi Sun 1, Li Yin 1 2, Jinyi Liu 1 The Olfactory Receptor Olfr25 Mediates Sperm Dysfunction Induced by Low-Dose Bisphenol A through the CatSper-Ca2+ Signaling Pathway Toxics, 12, 442 (2024)

PubMed: 38922122

Applications

WB

Reactivity

Mouse