BMP15 Antibody

• Catalog Number: orb377952
• Category: Antibodies
• Description: The BMP15 Antibody is suitable for IF, IHC, WB. It is a Polyclonal, Unconjugated antibody which raised against Recombinant fusion protein of human BMP15 .Purification: The antibody was purified by immunogen affinity chromatography.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: Recombinant fusion protein of human BMP15
• UniProt ID: Q9Z0L4, O95972
• Tested applications: IF, IHC, WB
• Dilution range: WB: 1:500-2000, IHC-P: 1:50-200
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: GDF9B; Bone morphogenetic protein 15; BMP-15; Grow
• Research Area: Epigenetics
• Note: For research use only
• Entrez: 12155, 9210

References

Knapczyk-Stwora, Katarzyna et al. Neonatal exposure to agonists and antagonists of sex steroid receptors induces changes in the expression of oocyte-derived growth factors and their receptors in ovarian follicles in gilts Theriogenology, 134, 42-52 (2019)

PubMed: 31132720

Western blot analysis of BMP15 expression in OVCAR3 (A), mouse kidney (B), mouse liver (C), rat brain (D) whole cell lysates. (Predicted band size: 45 kD; Observed band size: 54 kD)

Immunohistochemical analysis of BMP15 staining in rat kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of BMP15 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).