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Bcl-2/BCL2 Rabbit Polyclonal Antibody

SKU: orb402177

Description

The Bcl-2/BCL2 Rabbit Polyclonal Antibody is an unconjugated antibody raised in rabbit. It is immunogen affinity purified and suitable for use in ELISA, FC, ICC, IF, and WB applications. This antibody targets the Apoptosis regulator Bcl-2 in Human samples.

Research Area

Cancer Biology, Cell Biology, Metabolism Research, Signal Transduction

Images & Validation

Tested ApplicationsELISA, FC, ICC, IF, WB
Dilution RangeWestern blot, 0.1-0.5μg/ml, Human Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x10^6 cells, Human
ReactivityHuman
Application Notes
For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeRabbit IgG
ImmunogenE. coli-derived human Bcl-2 recombinant protein (Position: Q118-E165).
TargetApoptosis regulator Bcl-2
Molecular Weight26 kDa
PurificationImmunogen affinity purified.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLyophilized
Buffer/PreservativesEach vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
ConcentrationAdding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

Apoptosis regulator Bcl 2; B cell CLL/lymphoma 2; BCL2; BCL-2; Oncogene B cell leukemia 2

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UniProt Details

No UniProt data available

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
FC
Flow Cytometry
View Protocol
IF
Immunofluorescence
View Protocol
ICC
Immunocytochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

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100 μg
$ 470.00
Free Secondary Antibody (20 ul)0/0

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DispatchUsually dispatched within 2-4 weeks
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