5-HT5A Antibody

SKU: orb2649645

Description

Rabbit polyclonal antibody to 5-HT5A

Images & Validation

• Tested Applications: IF, WB
• Dilution Range: WB: WB (1/500 - 1/1000), IF/IC (1/100 - 1/500), IF: WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)
• Reactivity: Human, Rat

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Clone No.: HTR5A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

5-hydroxytryptamine receptor 5A; 5-HT-5; 5-HT-5A; 5-HT5A; Serotonin receptor 5A

Western blot analysis of 5-HT5A expression in rat kidney (A) whole cell lysates.

Immunofluorescent analysis of 5-HT5A staining in LOVO cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4°C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Quick Database Links

UniProt

P47898