Annexin IV Recombinant Mouse Monoclonal Antibody

SKU: orb1152027

Description

Annexin IV Recombinant Mouse Monoclonal Antibody

Research Area

Annexins, Cell Signaling

Images & Validation

• Tested Applications: ICC, IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:50-200, IF=1:100-500
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Recombinant
• Isotype: IgG
• Clone No.: BF6E9
• Immunogen: KLH conjugated synthetic peptide derived from human Annexin IV
• Target: ANXA4
• Molecular Weight: 32 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ANXA4_BOVIN; ANXA4; 35-beta calcimedin; Annexin IV; Annexin-4; Carbohydrate-binding protein p33/p41; Chromobindin-4; Endonexin I; Lipocortin IV; P32.5; PP4-X; Placental anticoagulant protein II (PAP-II); Protein II; ANX4; ANXA4_CANLF; 36 kDa zymogen granule membrane-associated protein (ZAP36); ANXA4_HUMAN; ANXA4_MOUSE; ANXA4_PIG; ANXA4_RAT;

Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:1000, Primary ab incubation condition: 4°C overnight, Lysate: HeLa, HepG2, SW480, SH-SY5Y, Protein loading quantity: 20 µg, Exposure time: 60 s, Predicted MW: 32 kDa, Observed MW: 32 kDa.

Cell line: HeLa, Fixative: 4% Paraformaldehyde, Permeabilization: 0.1% TritonX-100, Primary ab Dilution: 1:50, Primary incubation condition: 4°C overnight, Nuclear counter stain: DAPI (Blue), Comment: Color green is the positive signal for orb1152027.

Tissue: Human placenta, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:100, Primary ab incubation condition: 1 hour at room temperature, Counter stain: Hematoxylin, Comment: Color brown is the positive signal for orb1152027.

Quick Database Links

Gene Symbol

ANXA4

UniProt

P09525