Ankrd26 Antibody

SKU: orb345534

Description

Ankrd26 antibody

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:20,000 - 1:80,000, WB: 1:500 - 1:3,000
• Reactivity: Mouse
• Application Notes:
  This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 81 kDa in size corresponding to Ankrd26 by western blotting in the appropriate mouse tissue.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the N-Terminal region near amino acids 1-25 of mouse Ankrd26 protein.
• Target: Ankrd26
• Purity: This affinity-purified antibody is directed against mouse Ankrd26 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross reactivity with Ankrd26 proteins from mouse (100%), rat (93%), bovine (81%) and human (68%). Reactivity against homologues from other sources is not known.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.48 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-Ankrd26 Antibody, ankrd 26, ankrd-26, Ankyrin repeat domain 26 antibody, Ankyrin repeat domain containing protein 26 antibody, bA145E8.1 antibody, KIAA1074 antibody

Western blot using Biorbyt's affinity purified anti-Ankrd26 antibody shows detection of a band at ~81 kDa corresponding to mouse Ankrd26 protein. Lane 1 Blank, Lane 2 MES cell lysate - 80 µg, Lane 3 MES cell lysate - 40 µg, Lane 4 293T-ANKRD26 transfected cell lysate - 20 µg, Lane 5 control 293T cell lysate - 20 µg, Lane 6 BSA-ANKRD26 conjugate 20 ng, Lane 7 BSA - 500 ng, Lane 8 BSA - 100 ng, Lane 9 BSA 20 ng and Lane 10 Protein standards. Detection of endogenous Ankrd26 protein in MES cell lysates may occur when detection methods with higher sensitivity are used. Proteins were separated by SDS-PAGE, transferred to nitrocellulose, and probed with the primary antibody diluted to 1:1000 followed by detection using ALP conjugated Gt-a-Rabbit IgG (orb347655 is suggested) diluted to 1:3000. Size estimation was made by comparison to prestained MW markers as indicated.

Quick Database Links

Gene Symbol

Ankrd26

UniProt

Q811D2

RefSeq:28436723