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Alpha-2-Macroglobulin Antibody Peroxidase Conjugated
Catalog Number: orb344245
| Catalog Number | orb344245 |
|---|---|
| Category | Antibodies |
| Description | Alpha-2-Macroglobulin antibody (Peroxidase) |
| Species/Host | Goat |
| Clonality | Polyclonal |
| Tested applications | DOT, ELISA, IHC, WB |
| Reactivity | Human |
| Isotype | IgG |
| Immunogen | a2-Macroglobulin [Human Plasma] |
| Antibody Type | Primary Antibody |
| Concentration | 1.0 mg/ml |
| Dilution range | ELISA: 1:400 - 1:2,000, IHC: 1:50 - 1:250, WB: 1:100 - 1:500 |
| Form/Appearance | Lyophilized |
| Purity | Anti-Alpha 2-Macroglobulin is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified a2-Macroglobulin [Human Plasma]. Cross reactivity against a2-Macroglobulin from other sources is unknown. |
| Conjugation | HRP |
| UniProt ID | P01023 |
| NCBI | NP_000005.2 |
| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Buffer/Preservatives | 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! |
| Alternative names | goat anti-Alpha-2-Macroglobulin Antibody HRP Conju Read more... |
| Note | For research use only |
| Application notes | Anti-Alpha 2-Macroglobulin has been tested by dot blot and western blot and is suitable to be assayed against 1.0 ug of alpha-Macroglobulin [Human Plasma] in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:12,000 to 1:60,000 of the reconstitution concentration is suggested for this product. |
| Expiration Date | 12 months from date of receipt. |
Biorbyt Goat anti Alpha-2-Macroglobulin antibody was used to detect Alpha-2-Macroglobulin under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1 ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody (ON 4 C in orb348637). Detection shown was using Dylight 649 conjugated Donkey anti goat (1:10K in TBS/orb348637) 1 hr RT.
