ALKALINE PHOSPHATASE Antibody Biotin Conjugated

SKU: orb344773

Description

Alkaline Phosphatase antibody (Biotin)

Images & Validation

• Tested Applications: DOT, ELISA
• Dilution range: ELISA: User Optimized, WB: User Optimized
• Reactivity: Human
• Application Notes:
  Anti-Alkaline Phosphatase Biotin Antibody has been tested by ELISA and dot blot. This product is assayed against 1.0 ug of Alkaline Phosphatase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Alkaline Phosphatase [Human Intestine]
• Purity: Anti-Alkaline Phosphatase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Alkaline Phosphatase [Human Intestine]. Cross reactivity against Alkaline Phosphatase from other sources may occur but has not been specifically determined.

Storage & Handling

• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Concentration: 1 mg/mL
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-Alkaline Phosphatase Antibody biotin Conjugation, biotin conjugated rabbit anti-Alkaline phosphatase intestinal antibody, Alkaline phosphomonoesterase antibody, ALPI antibody, Glycerophosphatase antibody, IAP antibody, Intestinal alkaline phosphatase antibody, Kasahara isozyme antibody

Dot Blot for Anti-ALKALINE PHOSPHATASE (Human Intestine) (RABBIT) Antibody Biotin Conjugated. Lane 1: 100 ng Alkaline Phosphatase. Lanes 2-5: serial dilution 3 fold of Antigen. Primary Antibody: Anti-Alkaline Phosphatase Antibody Biotin Conjugated at 10 µg/ml at RT for 1 hour. Secondary Antibody: Streptavidin Peroxidase Conjugated (p/n orb348767) at a 1:40000 dilution at RT for 30 minutes. Block: orb348637 at RT for 30 minutes.

Quick Database Links

UniProt

P09923

RefSeq (Protein):NP_001622.2