ALP1 Antibody (Biotin)

SKU: orb344772

Description

Alkaline Phosphatase antibody (Biotin)

Images & Validation

• Tested Applications: DOT, ELISA
• Dilution Range: ELISA: User Optimized, WB: User Optimized
• Reactivity: Human
• Application Notes:
  Anti-Alkaline Phosphatase Biotin Antibody has been tested by ELISA and dot blot. This product is assayed against 1.0 ug of Alkaline Phosphatase in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product. Reconstitution Buffer: Restore with deionized water (or equivalent). Reconstitution Volume: 100 µL

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Alkaline Phosphatase [Human Intestine]
• Target: ALP1
• Purity: Anti-Alkaline Phosphatase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Alkaline Phosphatase [Human Intestine]. Cross reactivity against Alkaline Phosphatase from other sources may occur but has not been specifically determined.
• Conjugation: Biotin

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Rabbit anti-Alkaline Phosphatase Antibody biotin Conjugation, biotin conjugated rabbit anti-Alkaline phosphatase intestinal antibody, Alkaline phosphomonoesterase antibody, ALPI antibody, Glycerophosphatase antibody, IAP antibody, Intestinal alkaline phosphatase antibody, Kasahara isozyme antibody

Dot Blot for Anti-ALKALINE PHOSPHATASE (Human Intestine) (RABBIT) Antibody Biotin Conjugated. Lane 1: 100 ng Alkaline Phosphatase. Lanes 2-5: serial dilution 3 fold of Antigen. Primary Antibody: Anti-Alkaline Phosphatase Antibody Biotin Conjugated at 10 µg/ml at RT for 1 hour. Secondary Antibody: Streptavidin Peroxidase Conjugated (p/n orb348767) at a 1:40000 dilution at RT for 30 minutes. Block: orb348637 at RT for 30 minutes.

Quick Database Links

Gene Symbol

ALP1

UniProt

P09923

RefSeq (Protein):NP_001622.2