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Description
Research Area
Signal Transduction
Images & Validation
−
| Tested Applications | IF, IP, WB |
|---|---|
| Dilution Range | WB: 1:1000~1:2000, IF: 1:100~1:500, IP: 1:50~1:200 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | Synthetic phosphopeptide derived from human AKT around the phosphorylation site of Serine 473. |
| Molecular Weight | ~ 60 kDa |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE). |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH 7.2. |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−RAC-alpha serine/threonine-protein kinase; Protein kinase B; PKB; Protein kinase B alpha; PKB alpha; Proto-oncogene c-Akt; RAC-PK-alpha; AKT1; PKB, RAC
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Protocol Information
WB
Western Blot (IB, immunoblot)
IF
Immunofluorescence
IP
Immunoprecipitation
Available Sizes
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Free Secondary Antibody (20 ul)0/0
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Usually dispatched within 5-10 working days