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| Catalog Number | orb344777 |
|---|---|
| Category | Antibodies |
| Description | AKT antibody (Biotin) |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Isotype | IgG |
| Conjugation | Biotin |
| Reactivity | Human, Mouse, Rat |
| Form/Appearance | Lyophilized |
| Concentration | 1 mg/mL |
| Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
| Purity | AKT Antibody reacts with the AKT from human tissues. Anti-AKT antibody was purified from monospecific antiserum by Protein A chromatography. Based on sequence we expect this antibody to react as well with rat, mouse, and chicken AKT. |
| Immunogen | Anti-AKT Pan Reactive Antibody was produced in rabbits by repeated immunizations with a cocktail containing recombinant human AKT1, AKT2, and AKT3 protein isoforms. |
| UniProt ID | P31749 |
| Tested applications | IHC, IP, WB |
| Dilution range | IHC: 1:500 - 1:2,000, IP: 1:500 - 1:2,000, WB: 1:500 - 1:2,000 |
| Application notes | Biotin Conjugated AKT Antibody is tested for western blotting to detect a single band of the expected apparent molecular weight. This antibody is suitable for immunohistochemistry (Formalin-fixed paraffin-embedded sections), immunofluorescence microscopy using paraformaldehyde-fixed primary cardiomyocyte cultures, immunoprecipitation, transfected cell culture, primary cell culture and ELISA. Researchers should determine optimal titers for applications that are not stated below. |
| Antibody Type | Primary Antibody |
| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Alternative names | rabbit anti-AKT Antibody Biotin Conjugated, Biotin Read more... |
| Note | For research use only |
| NCBI | NP_001014431.1 |
| Expiration Date | 12 months from date of receipt. |
ELISA, IF, IHC, WB | |
Human, Monkey, Mouse, Rat | |
Mouse | |
Monoclonal | |
Biotin |
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