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AKAP5 Antibody
Catalog Number: orb1260100
| Catalog Number | orb1260100 |
|---|---|
| Category | Antibodies |
| Description | AKAP5 Antibody |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | IF, WB |
| Reactivity | Human, Mouse |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human AKAP5 (NP_004848.3). |
| Antibody Type | Primary Antibody |
| Concentration | batch dependent |
| Form/Appearance | Liquid |
| Conjugation | Unconjugated |
| MW | Observed: 47kDa |
| Target | AKAP5 |
| UniProt ID | P24588 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | Liquid |
| Alternative names | AKAP5, AKAP75, AKAP79, H21 Read more... |
| Note | For research use only |
| Application notes | WB: 1:500 - 1:2000IF: 1:50 - 1:200 |
| Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using AKAP5 Antibody (orb1260100) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunofluorescence analysis of HeLa cells using AKAP5 antibody (orb1260100) at dilution of 1:100. Blue: DAPI for nuclear staining.
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AKAP 79 rabbit pAb [orb764497]
ELISA, IF, IHC-P, WB
Human, Mouse, Rat
Polyclonal
Unconjugated
100 μl, 50 μl - Item 1 of 3
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Anti-AKAP5 Antibody [orb224054]
WB
Human, Mouse, Primate, Rat
Rabbit
Polyclonal
Unconjugated
200 μl, 100 μl, 30 μl
