AHA1 antibody

• Catalog Number: orb345001
• Category: Antibodies
• Description: AHA1 antibody
• Species/Host: Rat
• Clonality: Monoclonal
• Clone Number: 25F2.D9
• Tested applications: ELISA, IHC, WB
• Reactivity: Human
• Isotype: IgG2a
• Immunogen: This Protein G purified monoclonal antibody was produced in rats by repeated immunizations with full length recombinant mouse AHA1 protein followed by hybridoma development.
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:20,000, IHC: 5-10 µg/mL, WB: 1:1,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: This product is an IgG fraction antibody purified from tissue culture supernatant by Protein-G chromatography, followed by extensive dialysis against the buffer stated above. It is directed against human Aha1 protein. A BLAST analysis was used to suggest cross-reactivity with Aha1 protein from mouse, human and chimpanzee based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
• Conjugation: Unconjugated
• UniProt ID: Q8BK64
• NCBI: NP_666148.1
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: None
• Alternative names: rat anti-Aha1 Antibody, Aha1, Ahsa1 antibody, Acti
• Note: For research use only
• Application notes: This Protein-G purified antibody has been tested for use in ELISA, immunohistochemistry and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 38-40 kDa in size corresponding to Aha1 protein by western blotting in the appropriate cell lysate or extract.
• Expiration Date: 12 months from date of receipt.

Immunohistochemical staining of a 5-10 ug/mL to detect AHA1 in the seminiferous tubule of human testis (40X) showing moderate staining. Leydig cells showed faint to moderate staining using AHA1 antibody

Western blot analysis of A431 whole cell lysate (lane 1) and MCF-7 whole cell lysate (lane 2) using AHA1 antibody