ADORA2A/Adenosine A2A Receptor Antibody

SKU: orb1537749

Description

ADORA2A/Adenosine A2A Receptor Antibody

Images & Validation

• Tested Applications: FC, IHC, IHC-P, WB
• Dilution Range: Flo, IHC, IHC-P (5 µg/ml), IHC-Pfa (1 µg/ml), WB (1:1000)
• Reactivity: Canine, Guinea pig, Human, Mouse, Rabbit, Rat
• Application Notes:
  Further information: Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 5 ug/ml. Western Blot: 1:1000-1:5000 detected Adenosine Receptor A2A in HEK293 cells expressing canine A1/A2A receptor chimeras.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone No.: 7F6-G5-A2 / 7F6
• Immunogen: 6His-tagged full-length human recombinant A2A receptor. Epitope mapped to SQPLPGER in the third intracellular loop.
• Target: ADORA2A/Adenosine A2A Receptor
• Purification: Protein G purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide
• Concentration: 0.5 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ADORA2A, Adenosine receptor A2a, ADORA2, A2a receptor, Adenosine A2a receptor, Adenosine A2 receptor, Adenosine receptor subtype A2a, HA2aR, RDC8, AA2AR, A2AR

Quick Database Links

Gene Symbol

ADORA2A/Adenosine A2A Receptor