ADORA2A/Adenosine A2A Receptor Antibody (3rd Intracellular Domain, clone 7F6-G5-A2 / 7F6)

• Catalog Number: orb1537749
• Category: Antibodies
• Description: ADORA2A/Adenosine A2A Receptor Antibody (3rd Intracellular Domain, clone 7F6-G5-A2 / 7F6)
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 7F6-G5-A2 / 7F6
• Tested applications: FC, IHC, IHC-P, WB
• Reactivity: Canine, Guinea pig, Human, Mouse, Rabbit, Rat
• Isotype: IgG2a
• Immunogen: 6His-tagged full-length human recombinant A2A receptor. Epitope mapped to SQPLPGER in the third intracellular loop.
• Concentration: 0.5 mg/ml
• Dilution range: Flo, IHC, IHC-P (5 µg/ml), IHC-Pfa (1 µg/ml), WB (1:1000)
• Conjugation: Unconjugated
• Target: ADORA2A/Adenosine A2A Receptor
• Storage: Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
• Buffer/Preservatives: 0.01M Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide. 0.01M Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide
• Alternative names: ADORA2A, Adenosine receptor A2a, ADORA2, A2a recep
• Note: For research use only
• Application notes: Further information: Immunohistochemistry: LS-B2122 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2122 was determined to be 5 ug/ml. Western Blot: 1:1000-1:5000 detected Adenosine Receptor A2A in HEK293 cells expressing canine A1/A2A receptor chimeras.
• Expiration Date: 12 months from date of receipt.