ADHP

SKU: orb1219048

Description

ADHP is a fluorogenic peroxidase substrate (λex=530 nm, λem=590 nm).

Key Properties

• CAS Number: 119171-73-2
• MW: 257.24
• Purity: >98% (HPLC)
• Formula: C₁₄H₁₁NO₄
• SMILES: CC(N1C2=C(C=C(O)C=C2)OC3=CC(O)=CC=C13)=O
• Target: Others
• Solubility: In Vitro: DMSO : 100 mg/mL (388.74 mM)

Bioactivity

• In Vitro:
  To obtain the parameters Km and kcat for Compound I, two independent methods are used. Initially, the oxidation of ADHP using the injector functionality built-in to the fluorescence plate reader is studied. The auto-injector dispenses the H2O2 to initiate the reaction, as a means of generating a set of progress curves. Analysis for MPO-mediated oxidation of ADHP gives a Km of 31±4 μM and the kcat of 186± 6 s 1.The kobs also increases over the experimental range of ADHP concentrations from 1 to 80 μM and for the converse experiment holding substrate constant over 3 to 45 nM MPO. The apparent second order rate constant obtain from the slope of kobs against ADHP concentration Kappon is 2.1±0.2 mM/s.

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

10-Acetyl-3,7-dihydroxyphenoxazine

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