Adenosine Deaminase antibody

• Catalog Number: orb344597
• Category: Antibodies
• Description: Adenosine Deaminase antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IP, WB
• Reactivity: Bovine
• Isotype: IgG
• Immunogen: Adenosine Deaminase [Calf Spleen]
• Concentration: 1.0 mg/ml
• Dilution range: ELISA: 1:5,000 - 1:20,000, IP: 1:100, WB: 1:500 - 1:5,000
• Form/Appearance: Lyophilized
• Purity: Anti-Adenosine Deaminase antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Adenosine Deaminase [Calf Spleen]. Cross reactivity against Adenosine Deaminase from other tissues and species may occur but have not been specifically determined.
• Conjugation: Unconjugated
• UniProt ID: P56658
• NCBI: NP_776312.1
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-Adenosine Deaminase Antibody, Adenosin
• Note: For research use only
• Application notes: Anti-Adenosine Deaminase antibody has been tested in western blot and is suitable for ELISA and Immunoprecipitation applications. Anti-ADENOSINE DEAMINASE antibody was tested against 1.0 ug of Adenosine Deaminase [Calf Spleen] by immunoblot using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and DAB as a substrate at room temperature. A working dilution of 1:8,000 to 1:40,000 is suggested for this product.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of used to detect adenosine deaminase in mouse pancreas lysate (Left, Lane 1, 30 ul) under reducing conditions using Adenosine Deaminase antibody