ADAR Antibody

• Catalog Number: orb1256130
• Category: Antibodies
• Description: ADAR Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 150-250 of human ADAR (NP_001102.2).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 136kDa
• Target: ADAR
• UniProt ID: P55265
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: ADAR1, AGS6, DRADA, DSH, DSRAD, G1P1, IFI-4, IFI4,
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using ADAR antibody (orb1256130) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 20s.

Immunohistochemistry of paraffin-embedded rat testis using ADAR antibody (orb1256130) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat brain using ADAR antibody (orb1256130) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat heart using ADAR antibody (orb1256130) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human liver using ADAR antibody (orb1256130) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human breast cancer using ADAR antibody (orb1256130) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human stomach using ADAR antibody (orb1256130) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse testis using ADAR antibody (orb1256130) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse brain using ADAR antibody (orb1256130) at dilution of 1:100 (40x lens).

Immunoprecipitation analysis of 200 ug extracts of HeLa cells using 3 ug SUZ12 antibody (orb1274421). Western blot was performed from the immunoprecipitate using SUZ12 antibody (orb1274421) at a dilution of 1:500.

Immunofluorescence analysis of HeLa cells using ADAR antibody (orb1256130) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.