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ADAM17 Rabbit Polyclonal Antibody (FITC)

Catalog Number: orb7222

DispatchUsually dispatched within 2-3 weeks
$ 450.00
Catalog Numberorb7222
CategoryAntibodies
DescriptionADAM17 Rabbit Polyclonal Antibody (FITC)
TargetADAM17
ClonalityPolyclonal
Species/HostRabbit
IsotypeIgG
ConjugationFITC
ReactivityHuman, Mouse, Rat
Predicted ReactivityBovine, Canine, Porcine, Rabbit
Concentration1mg/ml
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
ImmunogenKLH conjugated synthetic peptide derived from human ADAM17 (201-300/824aa)
RRIDAB_10925992
Tested applicationsFC, IF
Antibody TypePrimary Antibody
StorageShipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Alternative namesA disintegrin and metalloproteinase domain 17; ADA
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NoteFor research use only
ADAM17 Rabbit Polyclonal Antibody (FITC)

Blank control: Molt4. Primary Antibody (green line): Rabbit Anti-ADAM17 antibody (orb7222), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

ADAM17 Rabbit Polyclonal Antibody (FITC)

Blank control: Molt4. Primary Antibody (green line): Rabbit Anti-ADAM17 antibody (orb7222), dilution: 3 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG, dilution: 1 µg/Test. Protocol, The cells wereincubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.