ADAM17 Rabbit Polyclonal Antibody

• Catalog Number: orb4374
• Category: Antibodies
• Description: ADAM17 Rabbit Polyclonal Antibody
• Target: ADAM17
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Porcine, Rabbit
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Immunogen: KLH conjugated synthetic peptide derived from human ADAM17 (201-300/824aa)
• UniProt ID: P78536
• RRID: AB_10931997
• MW: 68/91 kDa
• Tested applications: FC, ICC, IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, Flow-Cyt=1μg/Test, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ICC/IF=1:50-200
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: A disintegrin and metalloproteinase domain 17; ADA
• Note: For research use only

Blank control: U937 (fixed with 2% paraformaldehyde (10 min)). Primary Antibody: Rabbit Anti-ADAM17 antibody (orb4374), dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), dilution: 1:200 in 1X PBS containing 0.5% BSA.

Sample: HepG2 (Human) Cell Lysate at 30 ug, Primary: Anti-ADAM17 (orb4374) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 68/93 kD, Observed band size: 63/120 kD.

Sample: Testis (Mouse) Lysate at 40 ug, Primary: Anti-ADAM17 (orb4374) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 68/93 kD, Observed band size: 93/120 kD.