ADAM17 Rabbit Polyclonal Antibody

SKU: orb4374

Description

ADAM17 Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: FC, ICC, IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, Flow-Cyt=1μg/Test, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ICC/IF=1:50-200
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Porcine, Rabbit

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human ADAM17 (201-300/824aa)
• Target: ADAM17
• Molecular Weight: 68/91 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

A disintegrin and metalloproteinase domain 17; ADAM 17; ADAM metallopeptidase domain 17; ADAM17 protein; CD 156b; CD156b; CD156b antigen; CSVP; MGC71942; Snake venom like protease; TACE; TNF alpha convertase; TNF alpha converting enzyme; Tumor Necrosis Factor alpha Converting Enzyme; A disintegrin and metalloproteinase domain 17(tumor necrosis factor, alpha, converting enzyme); ADA17_HUMAN.

Blank control: U937 (fixed with 2% paraformaldehyde (10 min)). Primary Antibody: Rabbit Anti-ADAM17 antibody (orb4374), dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), dilution: 1:200 in 1X PBS containing 0.5% BSA.

Sample: HepG2 (Human) Cell Lysate at 30 ug, Primary: Anti-ADAM17 (orb4374) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 68/93 kD, Observed band size: 63/120 kD.

Sample: Testis (Mouse) Lysate at 40 ug, Primary: Anti-ADAM17 (orb4374) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 68/93 kD, Observed band size: 93/120 kD.

Quick Database Links

Gene Symbol

ADAM17

UniProt

P78536