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ACTN4 Antibody

SKU: orb1259060

Description

ACTN4 Antibody

Research Area

Cancer Biology, Cell Biology, Immunology & Inflammation, Signal Transduction

Images & Validation

Tested ApplicationsIF, WB
ReactivityHuman, Mouse, Rat
Application Notes
WB: 1:500 - 1:2000IF: 1:50 - 1:100

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 570-650 of human ACTN4 (NP_004915.2).
TargetACTN4
Molecular WeightObserved: 105kDa
PurificationAffinity purification
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesPBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Concentrationbatch dependent
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

ACTN4, DKFZp686K23158, FSGS, FSGS1, ACTININ-4

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ACTN4 Antibody

Western blot analysis of extracts of various cell lines, using ACTN4 antibody (orb1259060) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

UniProt Details

No UniProt data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IF
Immunofluorescence
View Protocol

ACTN4 Antibody (orb1259060)

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Available Sizes

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100 μl
$ 830.00
DispatchUsually dispatched within 5-10 working days
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