Alpha Actinin 4 Rabbit Polyclonal Antibody

SKU: orb10038

Description

Alpha Actinin 4 Rabbit Polyclonal Antibody

Research Area

Actin Crosslinking, Cancer Research, Cell Adhesion, Cell Biology, Hypoxia

Images & Validation

• Tested Applications: FC, IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=2ug/Test
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Gallus, Porcine, Rabbit

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Actinin alpha 4 (811-911/911aa)
• Target: ACTN4
• Molecular Weight: 105 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

actinin 4; Actinin alpha 4; Actinin alpha-4; alpha-actinin-4; α-Actinin-4; αActinin-4; actinin4; ACTN 4; ACTN4; ACTN4_HUMAN; DKFZp686K23158; F actin cross linking protein; F-actin cross-linking protein; Focal segmental glomerulosclerosis 1; FSGS 1; FSGS; FSGS1; Non muscle alpha actinin 4; Non-muscle alpha-actinin 4.

Blank control (black line): U251. Primary Antibody (green line): Rabbit Anti-alpha Actinin 4 antibody (orb10038), Dilution: 2 ug/Test, Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488, Dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Paraformaldehyde-fixed, paraffin embedded (Human breast carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (alpha Actinin 4-Loading Control) Polyclonal Antibody, Unconjugated (orb10038) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (alpha Actinin 4-Loading Control) Polyclonal Antibody, Unconjugated (orb10038) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Kidney (Rat) Lysate at 40 ug, Small intestine (Mouse) Lysate at 40 ug, Primary: Anti-alpha Actinin 4-Loading Control (orb10038) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 105 kD, Observed band size: 105 kD.

Sample: Lane 1: Mouse Uterus tissue lysates, Lane 2: Rat Uterus tissue lysates, Primary: Anti-alpha Actinin 4 (orb10038) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 105 kDa, Observed band size: 105 kDa.

Tissue/cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-alpha-Actinin-4 Polyclonal Antibody, Unconjugated (orb10038) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

ACTN4

UniProt

O43707