ACTH (7-23) Rabbit Polyclonal Antibody

SKU: orb10036

Description

ACTH (7-23) Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Guinea pig, Human, Porcine, Rabbit, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human ACTH (7-23/39aa)
• Target: POMC
• Molecular Weight: 4.3 kDa
• Purification: Affinity purified by Protein A

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

Adrenocorticotropic hormone; Adrenocorticotropin; Adrenocorticotropin Hormone; alpha Melanocyte Stimulating Hormone; beta Endorphin; beta Lipotropin; beta Melanocyte Stimulating Hormone; CLIP; Corticotropin; Corticotropin Like Intermediary Peptide; Corticotropin lipotropin precursor; Lipotropin beta; Lipotropin gamma; LPH; Melanotropin alpha; Met Enkephalin; MSH; NPP; POC; POMC; Pro opiomelanocortin; Proopiomelanocortin.

Sample: Lane 1: Pituitary (Mouse) Tissue Lysate, Lane 2: Pituitary (Rat) Tissue Lysate, Primary: Anti-ACTH (7-23) (orb10036) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 4.3 kD, Observed band size: 31/22/13 kD.

Tissue/cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-ACTH (7-23) Polyclonal Antibody, Unconjugated (orb10036) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

POMC

UniProt

P01189