ACTH (7-23) Rabbit Polyclonal Antibody

• Catalog Number: orb10036
• Category: Antibodies
• Description: ACTH (7-23) Rabbit Polyclonal Antibody
• Target: POMC
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Guinea pig, Human, Porcine, Rabbit
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Immunogen: KLH conjugated synthetic peptide derived from human ACTH (7-23/39aa)
• UniProt ID: P01189
• RRID: AB_10748484
• MW: 4.3 kDa
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Adrenocorticotropic hormone; Adrenocorticotropin;
• Note: For research use only

Sample: Lane 1: Pituitary (Mouse) Tissue Lysate, Lane 2: Pituitary (Rat) Tissue Lysate, Primary: Anti-ACTH (7-23) (orb10036) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 4.3 kD, Observed band size: 31/22/13 kD.

Tissue/cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-ACTH (7-23) Polyclonal Antibody, Unconjugated (orb10036) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.