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Catalog Number | orb248124 |
---|---|
Category | Antibodies |
Description | Acid phosphatases are a broad family of enzymes that differ widely in their amino acid length and homology, molecular weight and tissue of origin. They function to remove phosphate groups (or dephosphorylate) from proteins and perform best under acidic conditions. Disease states are sometimes characterized by abnormally high or low levels of Acid Phosphatase, leading to them being used as serological and histological disease markers. Prostatic Acid Phosphatase (PAP) was initially used as a prostate cancer marker but was later replaced with Prostate Serum Antigen (PSA), a more sensitive early stage cancer marker. |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | WB |
Reactivity | Human |
Isotype | Rabbit IgG |
Immunogen | The amino acid sequence used as immunogen for this antibody is specific to isoforms 2 (or B) and E of Acid Phosphatase. |
Dilution range | Western blot: 1-3ug/ml |
Purity | Protein A affinity chromatography |
Conjugation | Unconjugated |
Formula | 0.5 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide |
Hazard Information | This Acid Phosphatase antibody is available for research use only. |
Entrez | 52 |
Storage | Aliquot the Acid Phosphatase antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles. |
Buffer/Preservatives | 1X PBS, pH 7.4 |
Note | For research use only |
Application notes | Provided assay concentrations are suggestions only, Acid Phosphatase antibody titration may be required for optimal results. 1. This antibody is specific to isoforms 2 (or B) and E of Acid Phosphatase. |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of human samples using Acid Phosphatase (Isoform 2 specific) antibody (primary antibody dilution at: at 1 ug/ml)
Human | |
0.32-20 ng/mL | |
0.15 ng/mL |
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