Acid Phosphatase / ACP1 Antibody

• Catalog Number: orb248125
• Category: Antibodies
• Description: Acid phosphatases (AP) are a broad family of enzymes that differ widely in their AA length and homology, molecular weight and tissue of origin. They function to remove phosphate groups (or dephosphorylate) from proteins and perform best under acidic conditions. Disease states are sometimes characterized by abnormally high or low levels of Acid phosphatase, leading to them being used as serological and histological disease markers. Prostatic Acid Phosphatase (PAP) was initially used as a prostate cancer marker but was later replaced with Prostate Serum Antigen (PSA), a more sensitive early stage cancer marker.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: A recombinant protein fragment from the N-terminal portion of Acid Phosphatase / ACP1 was used as the immunogen for this antibody. This AA sequence is common to the A, C and D isoforms.
• Dilution range: Western blot: 1-3ug/ml
• Purity: Protein A affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.5 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This Acid Phosphatase / ACP1 antibody is available for research use only.
• Entrez: 52
• Storage: Aliquot the ACP1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: 1X PBS, pH 7.4
• Note: For research use only
• Application notes: Provided assay concentrations are suggestions only, ACP1 antibody titration may be required for optimal results. 1. This antibody will detect the A, C and D isoforms of Acid Phosphatase.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of human samples using ACP1 antibody (primary antibody dilution at: at 1 ug/ml)