H4K16ac Antibody

SKU: orb1257582

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Description

H4K16ac Antibody

Images & Validation

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Item 1 of 2

Tested Applications	ChIP, IF, WB
Reactivity	Human, Mouse, Rat
Application Notes	WB: 1:500 - 1:2000IF: 1:50 - 1:200ChIP: 1:50 - 1:200

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	A synthetic acetylated peptide around K16 of human Histone H4 (NP_001029249.1).
Target	H4K16ac
Molecular Weight	Observed: 13kDa
Purification	Affinity purification
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Concentration	batch dependent
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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H4/A, H4FA, HIST1H4B, H4/I, H4FI, HIST1H4C, H4/G, H4FG, HIST1H4D, H4/B, H4FB, HIST1H4E, H4/J, H4FJ, HIST1H4F, H4/C, H4FC, HIST1H4H, H4/H, H4FH, HIST1H4I, H4/M, H4FM, HIST1H4J, H4/E, H4FE, HIST1H4K, H4/D, H4FD, HIST1H4L, H4/K, H4FK, HIST2H4A, H4/N, H4F2, H4FN, HIST2H4, HIST2H4B, H4/O, H4FO, HIST4H4, Histone H4H4FA

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Western blot analysis of extracts of various cell lines, using Acetyl-Histone H4-K16 antibody (orb1257582) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunofluorescence analysis of C6 cells treated by TSA (upper left) and untreated C6 cells (upper right) using Acetyl-Histone H4-K16 Rabbit pAb (red, orb1257582) at dilution of 1:100. Blue: DAPI for nuclear staining.

Quick Database Links

Gene Symbol

H4K16ac

UniProt

P62805

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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Immunofluorescence

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ChIP

Chromatin Immunoprecipitation

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