Acetovanillone

SKU: orb1222169

Description

Acetovanillone is obtained from the rhizomes of several species of Apocynum; a selective inhibitor of NADPH oxidase.

Key Properties

• CAS Number: 498-02-2
• MW: 166.17
• Purity: >98% (HPLC)
• Formula: C₉H₁₀O₃
• SMILES: CC(=O)c1cc(c(cc1)O)OC
• Target: NADPH oxidase
• Solubility: DMSO : ≥ 100 mg/mL 601.79 mM; H2O : < 0.1 mg/mL

Bioactivity

• In Vivo:
  Animal model: SAMP6 mouse model (Pharmacokinetic assay). Dosage: 0.1 mg/kg/day. Administration: Intraperitoneal injection (i.p.), three times per week, for 3 months. Result: Showed a higher bone value and exhibited a lower percentage of SA-β-gal positive cells than the control group. Increased the expression of Ki67 and Oct-4 mRNA. Altered the osteoblast-osteoclast balance in bone and promoted the activity of osteoblasts. Animal model: Carrageenan (HY-125474)-induced pleurisy in male adult CD1 mice. Dosage: 5 mg/kg. Administration: Intraperitoneal injection (i.p.). Result: Blocked NADPH oxidase activation and attenuated neutrophil infiltration and lipid peroxidation in the lung tissue. Reduced the degree of PARP activation and the degree of IL-1b expression. Prevented carrageenan-induced IkB-a degradation and reduced the levels of NF-kB p65. Attenuated this iNOS expression, reduced the degree of positive staining for Fas ligand in the lung tissues. Inhibited cells apoptosis in carrageenan-treated mice. Prevented Bax expression and reduced the degree of positive staining for Bax. Attenuated carrageenan-induced inhibition of Bcl-2 expression and the loss of positive staining for Bcl-2 in mice subjected to carrageenan-induced pleurisy. Reduced the level of pERK1/2 and p38 expression.
• In Vitro:
  Western blot analysis. Cell line: Bone marrow stromal cells (BMSCs). Concentration: 100 μM. Incubation time: 1-7, 14 days. Result: Increased the expression levels of the “stemness markers” Nanog and Oct-4. Decreased the expression levels of p53, p21 and p16 at both the mRNA level and protein level. Increased the expression of sox-2 and klf-4 by 82.4% and 38.7%, respectively, compared with the negative control group. Reduced the expression of NADPH oxidase by 66.5% compared with the negative control. Had no change in the cell cycle or proliferation. Ddecreased the percentage of SA-β -gal-positive (green-stained) cells was by 42.5%. Increased the expression levels of four pivotal osteogenic markers (Runx2, OSX, Ocn, and Col1). Cell Proliferation Assay Cell line: HR-3Y1-2, 3Y1 cells. Concentration: 0, 1, 10, or 100 μM. Incubation time: 48h. Result: Inhibited the proliferation of HR-3Y1-2 but not 3Y1 cells at 10 μM and 100 μM. RT-PCR Cell line: HR-3Y1-2, 3Y1 cells. Concentration: 0, 1, 10, or 100 μM. Incubation time: 24, 36, 48 h. Result: Selectively down-regulated 1-integrin cell surface expression on the HR-3Y1-2 cells.

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Acetovanillone | NSC 209524

Quick Database Links

Gene Symbol

NADPH oxidase