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ACADM Antibody
SKU: orb1263636
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Description
Research Area
Cancer Biology, Metabolism Research, Signal Transduction
Images & Validation
−Item 1 of 4
| Tested Applications | FC, IF, IHC-P, WB |
|---|---|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Monkey |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit Ig |
| Immunogen | This ACADM antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 189-217 amino acids from the Central region of human ACADM. |
| Target | ACADM |
| Molecular Weight | 47 kDa |
| Purification | This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Supplied in PBS with 0.09% (W/V) sodium azide. |
| Concentration | batch dependent |
| Disclaimer | For research use only |
Alternative Names
−Medium-chain specific acyl-CoA dehydrogenase, mitochondrial, MCAD, ACADM
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Western blot analysis of ACADM Antibody in mouse heart tissue lysates (35 ug/lane)
Formalin-fixed and paraffin-embedded human colon carcinoma reacted with ACADM Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of widr cells using ACADM Antibody (bottom histogram) compared to a negative control cell (top histogram) FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Confocal immunofluorescent analysis of ACADM Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
FC
Flow Cytometry
IF
Immunofluorescence
ACADM Antibody (orb1263636)
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